Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients
MM van den Heuvel, DDC Vanhee, PE Postmus… - Journal of allergy and …, 1998 - Elsevier
Background: Several studies suggest a role for dendritic cells in the pathogenesis of allergic
disease. Objective: The purpose of this study was to compare function and phenotype of
monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control
subjects. Methods: MoDCs were developed by incubating adherent monocytes for 5 days
with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed
with flow cytometry, and the antigen-presenting function was assessed with the allogeneic …
disease. Objective: The purpose of this study was to compare function and phenotype of
monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control
subjects. Methods: MoDCs were developed by incubating adherent monocytes for 5 days
with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed
with flow cytometry, and the antigen-presenting function was assessed with the allogeneic …