Paternal imprinting of mouse serotonin receptor 2A gene Htr2 in embryonic eye: a conserved imprinting regulation on the RB/Rb locus.
MV Kato, Y Ikawa, Y Hayashizaki, H Shibata - 1998 - cabidigitallibrary.org
MV Kato, Y Ikawa, Y Hayashizaki, H Shibata
1998•cabidigitallibrary.orgParental allele-specific expression of the Htr2 gene was studied in tissues from reciprocal
crosses between C57BL/6J and PWK mice using the Sph 1 restriction site polymorphism
between the 2 mouse strains. Expression of the Htr2 gene in the cerebrum and ovaries was
demonstrated by reverse transcription (RT)-PCR analysis; digestion of the RT-PCR product
showed it to be the maternal allele. Expression in the embryonic eye was also exclusively
from the maternal allele.
crosses between C57BL/6J and PWK mice using the Sph 1 restriction site polymorphism
between the 2 mouse strains. Expression of the Htr2 gene in the cerebrum and ovaries was
demonstrated by reverse transcription (RT)-PCR analysis; digestion of the RT-PCR product
showed it to be the maternal allele. Expression in the embryonic eye was also exclusively
from the maternal allele.
Abstract
Parental allele-specific expression of the Htr2 gene was studied in tissues from reciprocal crosses between C57BL/6J and PWK mice using the Sph1 restriction site polymorphism between the 2 mouse strains. Expression of the Htr2 gene in the cerebrum and ovaries was demonstrated by reverse transcription(RT)-PCR analysis; digestion of the RT-PCR product showed it to be the maternal allele. Expression in the embryonic eye was also exclusively from the maternal allele.
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