Tyrosine kinase activation by the angiotensin II receptor in the absence of calcium signaling
TN Doan, MS Ali, KE Bernstein - Journal of Biological Chemistry, 2001 - ASBMB
The angiotensin II type 1 (AT 1) receptor signals via heterotrimeric G-proteins and
intracellular tyrosine kinases. Here, we investigate a modified AT 1 receptor, termed M5,
where the last five tyrosines (residues 292, 302, 312, 319, and 339) within the intracellular
carboxyl tail have been mutated to phenylalanine. This receptor did not elevate cytosolic
free calcium or inositol phosphate production in response to angiotensin II, suggesting an
uncoupling of the receptor from G-protein activation. Despite this, the M5 receptor still …
intracellular tyrosine kinases. Here, we investigate a modified AT 1 receptor, termed M5,
where the last five tyrosines (residues 292, 302, 312, 319, and 339) within the intracellular
carboxyl tail have been mutated to phenylalanine. This receptor did not elevate cytosolic
free calcium or inositol phosphate production in response to angiotensin II, suggesting an
uncoupling of the receptor from G-protein activation. Despite this, the M5 receptor still …
