Staphylococcus aureus Peptidoglycan Is a Toll-Like Receptor 2 Activator: a Reevaluation
R Dziarski, D Gupta - Infection and immunity, 2005 - Am Soc Microbiol
Infection and immunity, 2005•Am Soc Microbiol
Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently
questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble
Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction,
activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha
production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by
muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble
Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction,
activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha
production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by
muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
Abstract
Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
American Society for Microbiology