[HTML][HTML] Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages: enrichment of cathepsin H in early endosomes
V Claus, A Jahraus, T Tjelle, T Berg, H Kirschke… - Journal of biological …, 1998 - ASBMB
In this study we take advantage of recently developed methods using J774 macrophages to
prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well
as phagosomes of different ages enclosing 1-μm latex beads to investigate the steady state
distribution and trafficking of lysosomal enzyme activity between these organelles. At steady
state these cells appear to possess four different cellular structures, in addition to
phagolysosomes, where acid hydrolases were concentrated. The first site of hydrolase …
prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well
as phagosomes of different ages enclosing 1-μm latex beads to investigate the steady state
distribution and trafficking of lysosomal enzyme activity between these organelles. At steady
state these cells appear to possess four different cellular structures, in addition to
phagolysosomes, where acid hydrolases were concentrated. The first site of hydrolase …
