[HTML][HTML] Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice
Y Akamatsu, R Monroe, DD Dudley… - Proceedings of the …, 2003 - National Acad Sciences
The recombination-activating gene (RAG) 1 and RAG2 proteins comprise the lymphocyte-
specific components of the V (D) J recombinase and are required for the assembly of
antigen-receptor variable-region genes. A mutant truncated RAG2 protein (“core” RAG2)
lacking the C-terminal 144 amino acids, together with core RAG1, is able to mediate the
basic biochemical steps required for V (D) J recombination in vitro and in transfected cell
lines. Here we examine the effect of replacing the endogenous RAG2 locus in mice with …
specific components of the V (D) J recombinase and are required for the assembly of
antigen-receptor variable-region genes. A mutant truncated RAG2 protein (“core” RAG2)
lacking the C-terminal 144 amino acids, together with core RAG1, is able to mediate the
basic biochemical steps required for V (D) J recombination in vitro and in transfected cell
lines. Here we examine the effect of replacing the endogenous RAG2 locus in mice with …