Vaccine-Induced Protection against Helicobacter pylori in Mice Lacking Both Antibodies and Interleukin-4
CA Garhart, JG Nedrud, FP Heinzel… - Infection and …, 2003 - Am Soc Microbiol
CA Garhart, JG Nedrud, FP Heinzel, NE Sigmund, SJ Czinn
Infection and immunity, 2003•Am Soc MicrobiolTo test the hypothesis that a Th2 response to Helicobacter pylori is necessary for protection
and to address the possibility that humoral and Th2 cellular responses may compensate for
each other, we generated mice deficient in both interleukin-4 (IL-4) and antibodies. The
immunized double-knockout mice were protected from H. pylori challenge, as were the
parental strains and wild-type C57BL/6 mice. Neutralization of IL-4 in B-cell-deficient mice
did not prevent protection. Immunized IL-5-deficient mice were also protected. Thus, IL-4 …
and to address the possibility that humoral and Th2 cellular responses may compensate for
each other, we generated mice deficient in both interleukin-4 (IL-4) and antibodies. The
immunized double-knockout mice were protected from H. pylori challenge, as were the
parental strains and wild-type C57BL/6 mice. Neutralization of IL-4 in B-cell-deficient mice
did not prevent protection. Immunized IL-5-deficient mice were also protected. Thus, IL-4 …
Abstract
To test the hypothesis that a Th2 response to Helicobacter pylori is necessary for protection and to address the possibility that humoral and Th2 cellular responses may compensate for each other, we generated mice deficient in both interleukin-4 (IL-4) and antibodies. The immunized double-knockout mice were protected from H. pylori challenge, as were the parental strains and wild-type C57BL/6 mice. Neutralization of IL-4 in B-cell-deficient mice did not prevent protection. Immunized IL-5-deficient mice were also protected. Thus, IL-4 and IL-5 are not essential for protection.
American Society for Microbiology