Local knockdown of genes in the brain using small interfering RNA: a phenotypic comparison with knockout animals
A Salahpour, IO Medvedev, JM Beaulieu… - Biological …, 2007 - Elsevier
BACKGROUND: Recent reports have suggested effectiveness of RNA interference (RNAi)
for the analysis of gene functions in the brain. This study sought to determine the efficiency
of local small interfering RNA (siRNA) injections, comparing this approach with animals
generated through classical gene targeting. METHODS: Small interfering RNA against
dopamine transporter (DAT)(35 μg/14 days) or tyrosine hydroxylase (TH)(15 μg/3 days) was
injected into the ventral tegmental/substantia nigra areas of the brain of adult wildtype or …
for the analysis of gene functions in the brain. This study sought to determine the efficiency
of local small interfering RNA (siRNA) injections, comparing this approach with animals
generated through classical gene targeting. METHODS: Small interfering RNA against
dopamine transporter (DAT)(35 μg/14 days) or tyrosine hydroxylase (TH)(15 μg/3 days) was
injected into the ventral tegmental/substantia nigra areas of the brain of adult wildtype or …