Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen
CA Gleaves, TF Smith, EA Shuster… - Journal of clinical …, 1984 - Am Soc Microbiol
CA Gleaves, TF Smith, EA Shuster, GR Pearson
Journal of clinical microbiology, 1984•Am Soc MicrobiolA commercially available monoclonal antibody directed against an early nuclear protein of
cytomegalovirus was used with low-speed centrifugation for the rapid detection of this virus
from urine specimens inoculated onto MRC-5 cells. A total of 19 of 162 (11.7%) urine
specimens inoculated were positive by both immunofluorescence and peroxidase-
antiperoxidase procedures (sensitivity, 100%), whereas only 18 of the samples produced
cytopathic effects in conventional cell culture (specificity, 94.7%). All specimens were …
cytomegalovirus was used with low-speed centrifugation for the rapid detection of this virus
from urine specimens inoculated onto MRC-5 cells. A total of 19 of 162 (11.7%) urine
specimens inoculated were positive by both immunofluorescence and peroxidase-
antiperoxidase procedures (sensitivity, 100%), whereas only 18 of the samples produced
cytopathic effects in conventional cell culture (specificity, 94.7%). All specimens were …
A commercially available monoclonal antibody directed against an early nuclear protein of cytomegalovirus was used with low-speed centrifugation for the rapid detection of this virus from urine specimens inoculated onto MRC-5 cells. A total of 19 of 162 (11.7%) urine specimens inoculated were positive by both immunofluorescence and peroxidase-antiperoxidase procedures (sensitivity, 100%), whereas only 18 of the samples produced cytopathic effects in conventional cell culture (specificity, 94.7%). All specimens were positive by immunofluorescence and peroxidase-antiperoxidase procedures at 36 h postinfection, whereas an average of 9 days was required for cytopathic effects to develop in cell cultures.
American Society for Microbiology