Direct link from the suprachiasmatic nucleus to hypothalamic neurons projecting to the spinal cord: a combined tracing study using cholera toxin subunit B and …
N Vrang, JD Mikkelsen, PJ Larsen - Brain research bulletin, 1997 - Elsevier
By combining retrograde and anterograde tracing, evidence for a bineuronal connection
from the suprachiasmatic nucleus (SCN) to the intermediolateral cell column in the spinal
cord (IML) was obtained. The retrograde tracer cholera toxin subunit B (ChB) was pressure-
injected into the spinal cord and the anterograde tracer Phaseolus vulgaris-leucoagglutinin
(PHA-L) was iontophoretically injected into the SCN. The two tracers were visualized
simultaneously by a double immunohistochemical procedure. In the hypothalamus, ChB …
from the suprachiasmatic nucleus (SCN) to the intermediolateral cell column in the spinal
cord (IML) was obtained. The retrograde tracer cholera toxin subunit B (ChB) was pressure-
injected into the spinal cord and the anterograde tracer Phaseolus vulgaris-leucoagglutinin
(PHA-L) was iontophoretically injected into the SCN. The two tracers were visualized
simultaneously by a double immunohistochemical procedure. In the hypothalamus, ChB …