To carry out biochemical characterizations of human tyrosinase and to provide an unlimited
source of the enzyme for further study, an expression plasmid, pHis-Tyrosinase, which
contains the entire coding sequence except the signal sequence of a human tyrosinase was
constructed and expressed in Escherichia coli. The expressed enzyme was simply purified
by an immobilized metal affinity chromatography. The recombinant enzyme had the same
electrophoretic mobility as the native enzyme from human melanoma cell and cross-reacted …