Long-term self-renewable feeder-free human induced pluripotent stem cell–derived neural progenitors
Stem cells and development, 2011•liebertpub.com
Human induced pluripotent stem cells (hiPSCs) have led to an important revolution in stem
cell research and regenerative medicine. To create patient-specific neural progenitors
(NPs), we have established a homogenous, expandable, and self-renewable population of
multipotent NPs from hiPSCs, using an adherent system and defined medium supplemented
with a combination of factors. The established hiPSC-NPs highly expressed Nestin and
Sox1. These NPs were continuously propagated for∼ 1 year without losing their potential to …
cell research and regenerative medicine. To create patient-specific neural progenitors
(NPs), we have established a homogenous, expandable, and self-renewable population of
multipotent NPs from hiPSCs, using an adherent system and defined medium supplemented
with a combination of factors. The established hiPSC-NPs highly expressed Nestin and
Sox1. These NPs were continuously propagated for∼ 1 year without losing their potential to …
Human induced pluripotent stem cells (hiPSCs) have led to an important revolution in stem cell research and regenerative medicine. To create patient-specific neural progenitors (NPs), we have established a homogenous, expandable, and self-renewable population of multipotent NPs from hiPSCs, using an adherent system and defined medium supplemented with a combination of factors. The established hiPSC-NPs highly expressed Nestin and Sox1. These NPs were continuously propagated for ∼1 year without losing their potential to generate astrocytes, oligodendrocytes, and functional neurons and maintained a stable chromosome number. Voltage clamp analysis revealed outward potassium currents in hiPSC-NPs. The self-renewal characteristic of the NPs was demonstrated by a symmetrical mode of Nestin-positive cell division. Additionally, these hiPSC-NPs can be easily frozen and thawed in the presence of Rho-associated kinase (ROCK) inhibitor without losing their proliferation, karyotype stability, and developmental potential. The characteristics of our generated hiPSC-NPs provide the opportunity to use patient-specific or ready-to-use hiPSC-NPs in future biomedical applications.
Mary Ann Liebert