Analysis of inflammatory gene induction by oxidized phospholipids in vivo by quantitative real-time RT-PCR in comparison with effects of LPS
Oxidized phospholipids are thought to play a role in the development of atherosclerosis and
other chronic inflammatory processes. In this study, we analyzed the expression of
inflammatory genes induced by oxidized l-α-palmitoyl-2-arachidonoyl-sn-glycero-3-
phosphorylcholin (OxPAPC) in vitro and in vivo using quantitative real-time reverse
transcriptase-polymerase chain reaction (RT-PCR). Cultured human umbilical vein
endothelial cells (HUVEC) and monocyte-like U937 cells were treated with OxPAPC or …
other chronic inflammatory processes. In this study, we analyzed the expression of
inflammatory genes induced by oxidized l-α-palmitoyl-2-arachidonoyl-sn-glycero-3-
phosphorylcholin (OxPAPC) in vitro and in vivo using quantitative real-time reverse
transcriptase-polymerase chain reaction (RT-PCR). Cultured human umbilical vein
endothelial cells (HUVEC) and monocyte-like U937 cells were treated with OxPAPC or …
