Increased Interleukin-8 (II-8) in Rectal Dialysate From Patients With Ulcerative Colitis: Evidence for A Biological Role for IL-8 in Inflammation of The Colon
A Keshavarzian, RD Fusunyan, M Jacyno… - Official journal of the …, 1999 - journals.lww.com
A Keshavarzian, RD Fusunyan, M Jacyno, D Winship, RP MacDermott, IR Sanderson
Official journal of the American College of Gastroenterology| ACG, 1999•journals.lww.comOBJECTIVE: Infiltration of neutrophils and their release of toxic reactive oxygen species
(ROS) in the colonic mucosa are associated with tissue damage in ulcerative colitis (UC).
This neutrophil migration may be induced by chemoattractants, such as cytokines, in the
colonic milieu. One such chemoattractant is interleukin-8 (IL-8), a neutrophil chemokine that
is present at high concentrations in inflamed mucosa. However, the functional significance of
IL-8 in neutrophil attraction and activation in UC has not been established. We hypothesized …
(ROS) in the colonic mucosa are associated with tissue damage in ulcerative colitis (UC).
This neutrophil migration may be induced by chemoattractants, such as cytokines, in the
colonic milieu. One such chemoattractant is interleukin-8 (IL-8), a neutrophil chemokine that
is present at high concentrations in inflamed mucosa. However, the functional significance of
IL-8 in neutrophil attraction and activation in UC has not been established. We hypothesized …
Abstract
OBJECTIVE:
Infiltration of neutrophils and their release of toxic reactive oxygen species (ROS) in the colonic mucosa are associated with tissue damage in ulcerative colitis (UC). This neutrophil migration may be induced by chemoattractants, such as cytokines, in the colonic milieu. One such chemoattractant is interleukin-8 (IL-8), a neutrophil chemokine that is present at high concentrations in inflamed mucosa. However, the functional significance of IL-8 in neutrophil attraction and activation in UC has not been established. We hypothesized that IL-8 in the colonic lumen of patients with UC primes neutrophils, leading to their attraction and activation.
METHODS:
The colonic milieu was sampled by rectal dialysis. Using a semi-permeable membrane with a molecular weight cut-off of 12 kDa, dialysis solution was placed in the rectum and allowed to equilibrate over a 4-h period with the colonic milieu of controls or of patients with UC. IL-8 concentrations were measured by ELISA. Two functions of healthy neutrophils (PMN) were measured: expression of CD11-b surface adhesion molecules (by flow cytometry), and production of ROS (by both chemiluminescence and cytochrome C reduction assays). Neutrophil functions after exposure to rectal dialysates or n-formyl-methionyl-leucyl-phenylalanine (fMLP) were assessed before and after adding anti–IL-8 antibody or the fMLP blocker BMLP.
RESULTS:
IL-8 concentrations in dialysates from patients with active UC were significantly higher than in controls and correlated with disease activity. UC dialysates significantly increased ROS production and CD11-b expression by neutrophils and anti–IL-8 antibody partially (50%) inhibited these stimulatory effects of UC dialysates. Preincubation of neutrophils with UC dialysates significantly potentiated the fMLP-induced rise in ROS and anti–IL-8 antibody completely abolished this priming effect.
CONCLUSIONS:
The colonic milieu, sampled by rectal dialysis, from patients with active UC can both activate and prime neutrophils in vitro. High concentrations of IL-8 in the colonic lumen of UC patients are partially responsible for the activating effects of rectal dialysates, and account for all of its priming effects. These findings provide direct evidence for a role for IL-8 in inflammatory bowel disease.
Lippincott Williams & Wilkins