[HTML][HTML] Cerebrospinal Fluid Steroidomics: Are Bioactive Bile Acids Present in Brain? 2
M Ogundare, S Theofilopoulos, A Lockhart… - Journal of Biological …, 2010 - ASBMB
In this study we have profiled the free sterol content of cerebrospinal fluid by a combination
of charge tagging and liquid chromatography-tandem mass spectrometry. Surprisingly, the
most abundant cholesterol metabolites were found to be C 27 and C 24 intermediates of the
bile acid biosynthetic pathways with structures corresponding to 7α-hydroxy-3-oxocholest-4-
en-26-oic acid (7.170±2.826 ng/ml, mean±SD, six subjects), 3β-hydroxycholest-5-en-26-oic
acid (0.416±0.193 ng/ml), 7α, x-dihydroxy-3-oxocholest-4-en-26-oic acid (1.330±0.543 …
of charge tagging and liquid chromatography-tandem mass spectrometry. Surprisingly, the
most abundant cholesterol metabolites were found to be C 27 and C 24 intermediates of the
bile acid biosynthetic pathways with structures corresponding to 7α-hydroxy-3-oxocholest-4-
en-26-oic acid (7.170±2.826 ng/ml, mean±SD, six subjects), 3β-hydroxycholest-5-en-26-oic
acid (0.416±0.193 ng/ml), 7α, x-dihydroxy-3-oxocholest-4-en-26-oic acid (1.330±0.543 …