[HTML][HTML] Granzyme B mimics apical caspases: description of a unified pathway for trans-activation of executioner caspase-3 and-7
Granzyme B (GrB) is predicted to trigger apoptosis by activating preferred caspases, but the
zymogens that are directly processed by the granzyme and the requirements for these
interactions remain unclarified. We examined this dilemma by comparing the kinetics and
pattern of GrB-mediated activation of the executioner caspase-7 in vitro and in vivo. GrB
rapidly activates procaspase-7 in vitro by cleaving between the large and small subunits
leaving the propeptide intact. During GrB-mediated apoptosis, the caspase-7 propeptide is …
zymogens that are directly processed by the granzyme and the requirements for these
interactions remain unclarified. We examined this dilemma by comparing the kinetics and
pattern of GrB-mediated activation of the executioner caspase-7 in vitro and in vivo. GrB
rapidly activates procaspase-7 in vitro by cleaving between the large and small subunits
leaving the propeptide intact. During GrB-mediated apoptosis, the caspase-7 propeptide is …
