Leukoreduction system chambers are an efficient, valid, and economic source of functional monocyte-derived dendritic cells and lymphocytes
IA Pfeiffer, E Zinser, E Strasser, MF Stein, J Dörrie… - Immunobiology, 2013 - Elsevier
The demand for human monocyte-derived dendritic cells (moDCs), as well as for primary
human B and T lymphocytes for immunological research purposes has been increased in
recent years. Classically, these monocytes are isolated from blood, leukapheresis products
or buffy coats of healthy donors by plastic adherence of peripheral blood mononuclear cells
(PBMCs), followed by stimulation with granulocyte macrophage colony-stimulating factor
(GM-CSF) and interleukin (IL)-4, while lymphocytes are usually isolated from the non …
human B and T lymphocytes for immunological research purposes has been increased in
recent years. Classically, these monocytes are isolated from blood, leukapheresis products
or buffy coats of healthy donors by plastic adherence of peripheral blood mononuclear cells
(PBMCs), followed by stimulation with granulocyte macrophage colony-stimulating factor
(GM-CSF) and interleukin (IL)-4, while lymphocytes are usually isolated from the non …