[HTML][HTML] Attenuation of mammalian target of rapamycin activity by increased cAMP in 3T3-L1 adipocytes
PH Scott, JC Lawrence - Journal of Biological Chemistry, 1998 - ASBMB
Incubating 3T3-L1 adipocytes with forskolin, which increases intracellular cAMP by
activating adenylate cyclase, mimicked rapamycin by attenuating the effect of insulin on
stimulating the phosphorylation of four (S/T) P sites in PHAS-I, a downstream target of the
mammalian target of rapamycin (mTOR) signaling pathway. To investigate the hypothesis
that increasing cAMP inhibits mTOR, the protein kinase activity of mTOR was measured in
an immune complex assay with recombinant PHAS-I as substrate. Both forskolin and 8-(4 …
activating adenylate cyclase, mimicked rapamycin by attenuating the effect of insulin on
stimulating the phosphorylation of four (S/T) P sites in PHAS-I, a downstream target of the
mammalian target of rapamycin (mTOR) signaling pathway. To investigate the hypothesis
that increasing cAMP inhibits mTOR, the protein kinase activity of mTOR was measured in
an immune complex assay with recombinant PHAS-I as substrate. Both forskolin and 8-(4 …
