[HTML][HTML] Cell-free DNA: an upcoming biomarker in transplantation

EM Gielis, KJ Ledeganck, D Winter, J Del Favero… - American Journal of …, 2015 - Elsevier
After organ transplantation, donor-derived cell-free DNA (ddcfDNA) can be detected in the
recipient's blood and urine. Different ddcfDNA quantification techniques have been
investigated but a major breakthrough was made with the introduction of digital droplet PCR
and massive parallel sequencing creating the opportunity to increase the understanding of
ddcfDNA kinetics after transplantation. The observations of increased levels of ddcfDNA
during acute rejection and even weeks to months before histologic features of graft rejection …