Psoriatic T cells recognize neolipid antigens generated by mast cell phospholipase delivered by exosomes and presented by CD1a

KL Cheung, R Jarrett, S Subramaniam… - Journal of Experimental …, 2016 - rupress.org
KL Cheung, R Jarrett, S Subramaniam, M Salimi, D Gutowska-Owsiak, YL Chen, C Hardman…
Journal of Experimental Medicine, 2016rupress.org
Psoriasis is a chronic inflammatory skin disease associated with a T helper 17 response.
Yet, it has proved challenging to identify relevant peptide-based T cell antigens. Antigen-
presenting Langerhans cells show a differential migration phenotype in psoriatic lesions and
express constitutively high levels of CD1a, which presents lipid antigens to T cells. In
addition, phospholipase A2 (PLA2) is highly expressed in psoriatic lesions and is known to
generate neolipid skin antigens for recognition by CD1a-reactive T cells. In this study, we …
Psoriasis is a chronic inflammatory skin disease associated with a T helper 17 response. Yet, it has proved challenging to identify relevant peptide-based T cell antigens. Antigen-presenting Langerhans cells show a differential migration phenotype in psoriatic lesions and express constitutively high levels of CD1a, which presents lipid antigens to T cells. In addition, phospholipase A2 (PLA2) is highly expressed in psoriatic lesions and is known to generate neolipid skin antigens for recognition by CD1a-reactive T cells. In this study, we observed expression of a cytoplasmic PLA2 (PLA2G4D) in psoriatic mast cells but, unexpectedly, also found PLA2G4D activity to be extracellular. This was explained by IFN-α–induced mast cell release of exosomes, which transferred cytoplasmic PLA2 activity to neighboring CD1a-expressing cells. This led to the generation of neolipid antigens and subsequent recognition by lipid-specific CD1a-reactive T cells inducing production of IL-22 and IL-17A. Circulating and skin-derived T cells from patients with psoriasis showed elevated PLA2G4D responsiveness compared with healthy controls. Overall, these data present an alternative model of psoriasis pathogenesis in which lipid-specific CD1a-reactive T cells contribute to psoriatic inflammation. The findings suggest that PLA2 inhibition or CD1a blockade may have therapeutic potential for psoriasis.
rupress.org