[HTML][HTML] Establishment and characterization of cultured epithelial cells lacking expression of ZO-1
K Umeda, T Matsui, M Nakayama, K Furuse… - Journal of Biological …, 2004 - ASBMB
In well polarized epithelial cells, closely related ZO-1 and ZO-2 are thought to function as
scaffold proteins at tight junctions (TJs). In epithelial cells at the initial phase of polarization,
these proteins are recruited to cadherin-based spotlike adherens junctions (AJs). As a first
step to clarify the function of ZO-1, we successfully generated mouse epithelial cell clones
lacking ZO-1 expression (ZO-1-/-cells) by homologous recombination. Unexpectedly, in
confluent cultures, ZO-1-/-cells were highly polarized with well organized AJs/TJs, which …
scaffold proteins at tight junctions (TJs). In epithelial cells at the initial phase of polarization,
these proteins are recruited to cadherin-based spotlike adherens junctions (AJs). As a first
step to clarify the function of ZO-1, we successfully generated mouse epithelial cell clones
lacking ZO-1 expression (ZO-1-/-cells) by homologous recombination. Unexpectedly, in
confluent cultures, ZO-1-/-cells were highly polarized with well organized AJs/TJs, which …